Conference Day Two

Tuesday, 26 September

8:00 am Check In & Refreshments

8:45 am Chair’s Opening Remarks

Supercharging Advanced Characterisation Strategies to Translate Structural Data into Functionality Understanding

9:00 am Panel Discussion: Aligning LNP Formulative Innovation with Advanced Analytical Development

Synopsis

  • Evolving the perspective of LNP characterisation to keep pace with the development of next-generation LNPs
  • Reviewing the current standard of analytical technologies to adequately quantify LNP structure
  • Foreseeing the structural components of LNPs which will require more precise quantification to drive characterisation data to the next level  

9:15 am Spearheading Novel Approaches to Unravel Mechanisms of LNP Interactions with Endosomal Membranes

Synopsis

  • Providing new information about physiological interactions that occur at the site of delivery
  • Resolving mechanistic information about LNP interactions at the site to understand the full delivery journey
  • Looking towards evolving LNP design to align with the endosomal release required at the site of delivery

9:45 am Outlining Advanced Technology Available to Increase Accuracy of Traditional CQA Touchpoints

Synopsis

  • Moving beyond size, shape, zeta potential and encapsulation efficiency to unlock next-generation CQA assessments
  • Relaying CQA data to inform adaptations to manufacturing, evolving the process design
  • Driving advanced CQA assessment beyond traditional analytical touchpoints to evolve structural understanding

10:15 am Spearheading Novel Approaches to Unravel Mechanisms of LNP Interactions with Endosomal Membranes

Synopsis

  • Providing new information about physiological interactions that occur at the site of delivery
  • Resolving mechanistic information about LNP interactions at the site to understand the full delivery journey
  • Looking towards evolving LNP design to align with the endosomal release required at the site of delivery

10:45 am Lipid-based Nanoparticles: Spatially Resolved DLS as PAT for Inline Nanoparticle Size Characterization and Process Control in Manufacturing

11:00 am Morning Refreshments & Speed Networking

Addressing the Challenges Surrounding Stability Profiling to Ensure Formulation & Manufacturing Processes Protect the LNP Integrity

11:30 am Sharing Cutting-Edge Techniques Implemented to Ensure Stability of LNP Remains Central to Pre-Clinical Studies

  • Rongjun Chen Professor of Biomaterials Engineering, Imperial College London

Synopsis

Brand New Data!

  • Highlighting the structural characteristics of lipids, conjugates and payloads to develop a cohesive and stable LNP
  • Uncovering the interplay between charge dynamics and stability
  • Enhancing potency over time whilst establishing standardised LNP stability profiles

12:00 pm Lipid-based DNA delivery: towards alternative nucleic acid payloads

Synopsis

Brand New Data!

  • Discussing the promises and challenges of DNA payloads as an alternative to mRNA
  • Suggesting differences in LNP design when encapsulating different nucleic acid payloads and how the Hermes non-viral delivery platform has been optimized for synthetic DNA payloads
  • Leveraging the advantages of synthetic DNA payloads for vaccine and personalized medicine applications

12:30 pm An mRNA-LNP Vaccine Platform Tailoring the Immunogenicity Profile from Prophylactic to Therapeutic Applications, Reducing Reactogenicity and Extending Shelf-Life Stability

Synopsis

  • Addressing shortcomings in the immunogenicity, reactogenicity and stability of current mRNA vaccines by optimizing the lipid nanoparticle components
  • Investigating the addition of targeting lipids to tune the immunogenicity profile towards the requirements of different prophylactic and therapeutic applications
  • Extending the shelf-life of mRNA vaccines by developing lyophilizable LNPs and microneedle-array patches

1:00 pm Scalable Lipid Nanoparticles Formulation using Impingement Jets Mixing Systems

Synopsis

  • Scalability of impingement jets mixing (IJM) systems
  • Progress in automation of screening
  • Data from application partners – scaling up and out

1:15 pm Lunch & Networking

Revealing the Next Generation of LNP Design & Production to Innovate Current Measures of Development

2:15 pm Outlining Next-Generation Perspectives Across Innovative LNP Design

Synopsis

  • Reviewing fundamental strategies for extrahepatic targeting, addressing a range of disease indications
  • Highlighting the inherent challenges associated with biomolecular corona studies of LNPs
  • Questioning whether blebs and empty LNPs can be classified as positive or negative attributes

2:45 pm Meeting Review: The LNP White Space: What Does the Future Hold?

Synopsis

Unite as a community in an ultimate opportunity to celebrate the lessons learned over the past 3 days:

  • What are the key takeaways from the presentations and discussions at the summit?
  • How do we work more collaboratively as a community to drive progress?
  • Where should we be this time next year to classify as a successful year?

3:30 pm Chair’s Closing Remarks

3:45 pm End of Day Two & Close of Summit